| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MID1 (478-667) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MID1 |
| Gene ID: |
4281 |
| Uniprot ID: |
TRI18_HUMAN |
| Immunogen Region: |
478-667 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1). |
| Immunogen Sequence: |
KLKTNSQPFKLDPKSAHRKL KVSHDNLTVERDESSSKKSH TPERFTSQGSYGVAGNVFID SGRHYWEVVISGSTWYAIGL AYKSAPKHEWIGKNSASWAL CRCNNNWVVRHNSKEIPIEP APHLRRVGILLDYDNGSIAF YDALNSIHLYTFDVAFAQPV CPTFTVWNKCLTIITGLPIP DHLDCTEQLP |
| Tissue Specificity | In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain. |
| Post Translational Modifications | Phosphorylated on serine and threonine residues. |
| Function | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. |
| Protein Name | E3 Ubiquitin-Protein Ligase Midline-1MidinPutative Transcription Factor XprfRing Finger Protein 59Ring Finger Protein Midline-1Ring-Type E3 Ubiquitin Transferase Midline-1Tripartite Motif-Containing Protein 18 |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmCytoskeletonSpindleMicrotubule-AssociatedIt Is Associated With Microtubules Throughout The Cell CycleCo-Localizing With Cytoplasmic Fibers In Interphase And With The Mitotic Spindle And Midbodies During Mitosis And Cytokinesis |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Midline-1 antibodyAnti-Midin antibodyAnti-Putative Transcription Factor Xprf antibodyAnti-Ring Finger Protein 59 antibodyAnti-Ring Finger Protein Midline-1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Midline-1 antibodyAnti-Tripartite Motif-Containing Protein 18 antibodyAnti-MID1 antibodyAnti-FXY antibodyAnti-RNF59 antibodyAnti-TRIM18 antibodyAnti-XPRF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
