Host: | Rabbit |
Applications: | WB/IF/ICC/ELISA |
Reactivity: | MERS-CoV |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Immunogen Region: | 18-725 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-725 of coronavirus Spike RBD (YP_009047204.1). |
Immunogen Sequence: | YVDVGPDSVKSACIEVDIQQ TFFDKTWPRPIDVSKADGII YPQGRTYSNITITYQGLFPY QGDHGDMYVYSAGHATGTTP QKLFVANYSQDVKQFANGFV VRIGAAANSTGTVIISPSTS ATIRKIYPAFMLGSSVGNFS DGKMGRFFNHTLVLLPDGCG TLLRAFYCILEPRSGNHCPA GNSYTSFATYHTPATDCSDG NYNRNASLNSFKEYFNLRNC TFMYTYNITEDEILEWFGI |
Background | It s been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Information sourced from Uniprot.org