| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MCM3AP |
| Gene ID: | 8888 |
| Uniprot ID: | GANP_HUMAN |
| Immunogen Region: | 250-550 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human MCM3AP (NP_003897.2). |
| Immunogen Sequence: | SSFPVSSAVLGEPFQASKAG VRQGCEEAVSQVEPLPSLMK GLKRKEDQDRSPRRHGHEPA EDSDPLSRGDHPPDKRPVRL NRPRGGTLFGRTIQDVFKSN KEVGRLGNKEAKKETGFVES AESDHMAIPGGNQSVLAPSR IPGVNKEEETESREKKEDSL RGTPARQSNRSESTDSLGGL SPSEVTAIQCKNIPDYLNDR TILENHFGKIAKVQRIFTRR SKKLAVVHFFDHASAALAR |
| Tissue Specificity | Widely expressed. Up-regulated in germinal center B-cells in tonsils (at protein level). |
| Function | Isoform GANP: As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations. Isoform MCM3AP: Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations. |
| Protein Name | Germinal-Center Associated Nuclear Protein Ganp 80 Kda Mcm3-Associated Protein Mcm3 Acetylating Protein Mcm3ap Mcm3 Acetyltransferase |
| Cellular Localisation | Isoform Ganp: Nucleus Envelope Nucleus Nuclear Pore Complex Nucleoplasm Chromosome Predominantly Located At The Nuclear Envelope Facing The Nucleus Interior Localization At The Nuclear Pore Complex Requires Nup153 Tpr And Alyref/Aly Also Found Associated With Chromatin In B-Cells Targeted To The Immunoglobulin Variable Region Genes Isoform Mcm3ap: Cytoplasm Translocates Into The Nucleus In The Presence Of Mcm3 Associates With Chromatin Possibly Through Interaction With Mcm3 |
| Alternative Antibody Names | Anti-Germinal-Center Associated Nuclear Protein antibody Anti-Ganp antibody Anti-80 Kda Mcm3-Associated Protein antibody Anti-Mcm3 Acetylating Protein antibody Anti-Mcm3ap antibody Anti-Mcm3 Acetyltransferase antibody Anti-MCM3AP antibody Anti-GANP antibody Anti-KIAA0572 antibody Anti-MAP80 antibody |
Information sourced from Uniprot.org