• STJ72310 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72310 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-LY6E-Mouse antibody (Internal) (STJ72310)

SKU:
STJ72310-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC/IHC
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-LY6E-Mouse (Internal) is suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the membrane of NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Immunogen Region: Internal
Accession Number: NP_002337.1; NP_032555.1
Specificity: Reported variants represent identical protein: NP_002337.1, NP_001120685.1
Immunogen Sequence: SCQEKDHYCIT
Function GPI-anchored cell surface protein that regulates T-lymphocytes proliferation, differentiation, and activation. Regulates the T-cell receptor (TCR) signaling by interacting with component CD3Z/CD247 at the plasma membrane, leading to CD3Z/CD247 phosphorylation modulation. Restricts the entry of murine coronavirus, mouse hepatitis virus, by interfering with spike protein-mediated membrane fusion. Plays also an essential role in placenta formation by acting as the main receptor for syncytin-A (SynA). Therefore, participates in the normal fusion of syncytiotrophoblast layer I (SynT-I) and in the proper morphogenesis of both fetal and maternal vasculatures within the placenta. May also act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-3:beta-4-containing nAChRs maximum response.
Protein Name Lymphocyte Antigen 6e
Ly-6e
Stem Cell Antigen 2
Thymic Shared Antigen 1
Tsa-1
Database Links Reactome: R-MMU-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Lymphocyte Antigen 6e antibody
Anti-Ly-6e antibody
Anti-Stem Cell Antigen 2 antibody
Anti-Thymic Shared Antigen 1 antibody
Anti-Tsa-1 antibody
Anti-Ly6e antibody
Anti-Ly67 antibody
Anti-Sca-2 antibody
Anti-Tsa-1 antibody

Information sourced from Uniprot.org

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