| Post Translational Modifications | O- and N-glycosylated.some of the 18 N-linked glycans are polylactosaminoglycans. (Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells. |
| Function | Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. |
| Protein Name | Lysosome-Associated Membrane Glycoprotein 1Lamp-1Lysosome-Associated Membrane Protein 1Cd107 Antigen-Like Family Member ACd Antigen Cd107a |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Lysosome MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneLate Endosome MembraneCell MembraneCytolytic Granule MembraneThis Protein Shuttles Between LysosomesEndosomesAnd The Plasma MembraneColocalizes With Osbpl1a At The Late Endosome |
| Alternative Antibody Names | Anti-Lysosome-Associated Membrane Glycoprotein 1 antibodyAnti-Lamp-1 antibodyAnti-Lysosome-Associated Membrane Protein 1 antibodyAnti-Cd107 Antigen-Like Family Member A antibodyAnti-Cd Antigen Cd107a antibodyAnti-LAMP1 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368182/STJ11104366_1__89933.1713133691.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HeLa cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368183/STJ11104366_2__95394.1713133692.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368184/STJ11104366_3__35038.1713133692.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western blot analysis of lysates from U-937, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177010/368185/STJ11104366_4__39856.1713133693.jpg?c=1 "Western blot analysis of lysates from U-937, using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177010/368182/STJ11104366_1__89933.1713133691.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KD Validated] LAMP1 Rabbit polyclonal antibody (STJ11104366) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
