• Immunofluorescence analysis of rat bronchus using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse bronchus using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human lung cancer using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytokeratin 7 (KRT7) in paraffin-embedded human lung cancer using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat lung, using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of lysates from HepG2 cells, using Cytokeratin 7 (KRT7) Rabbit polyclonal antibody (STJ118022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-KRT7 antibody (1-94) (STJ118022)

SKU:
STJ118022

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT7 (1-94) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT7
Gene ID: 3855
Uniprot ID: K2C7_HUMAN
Immunogen Region: 1-94
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human Cytokeratin 7 (KRT7) (NP_005547.3).
Immunogen Sequence: MSIHFSSPVFTSRSAAFSGR GAQVRLSSARPGGLGSSSLY GLGASRPRVAVRSAYGGPVG AGIREVTINQSLLAPLRLDA DPSLQRVRQEESEQ
Tissue Specificity Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Post Translational Modifications Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Protein Name Keratin - Type Ii Cytoskeletal 7
Cytokeratin-7
Ck-7
Keratin-7
K7
Sarcolectin
Type-Ii Keratin Kb7
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 7 antibody
Anti-Cytokeratin-7 antibody
Anti-Ck-7 antibody
Anti-Keratin-7 antibody
Anti-K7 antibody
Anti-Sarcolectin antibody
Anti-Type-Ii Keratin Kb7 antibody
Anti-KRT7 antibody
Anti-SCL antibody

Information sourced from Uniprot.org

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