• Immunofluorescence analysis of U2OS cells using KPNA4 antibody (STJ24342). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using KPNA4 Antibody (STJ24342) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using KPNA4 Antibody (STJ24342) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of A-549, using KPNA4 Rabbit polyclonal antibody (STJ24342) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-KPNA4 antibody (1-240) (STJ24342)

SKU:
STJ24342

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KPNA4 (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KPNA4
Gene ID: 3840
Uniprot ID: IMA3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA4 (NP_002259.1).
Immunogen Sequence: MADNEKLDNQRLKNFKNKGR DLETMRRQRNEVVVELRKNK RDEHLLKRRNVPHEDICEDS DIDGDYRVQNTSLEAIVQNA SSDNQGIQLSAVQAARKLLS SDRNPPIDDLIKSGILPILV HCLERDDNPSLQFEAAWALT NIASGTSEQTQAVVQSNAVP LFLRLLHSPHQNVCEQAVWA LGNIIGDGPQCRDYVISLGV VKPLLSFISPSIPITFLRNV TWVMVNLCRHKDPPPPMET
Tissue Specificity Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Protein Name Importin Subunit Alpha-3
Importin Alpha Q1
Qip1
Karyopherin Subunit Alpha-4
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-168276
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Importin Subunit Alpha-3 antibody
Anti-Importin Alpha Q1 antibody
Anti-Qip1 antibody
Anti-Karyopherin Subunit Alpha-4 antibody
Anti-KPNA4 antibody
Anti-QIP1 antibody

Information sourced from Uniprot.org

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