| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IZUMO1 (1-350) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IZUMO1 |
| Gene ID: |
284359 |
| Uniprot ID: |
IZUM1_HUMAN |
| Immunogen Region: |
1-350 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human IZUMO1 (NP_872381.2). |
| Immunogen Sequence: |
MGPHFTLLCAALAGCLLPAE GCVICDPSVVLALKSLEKDY LPGHLDAKHHKAMMERVENA VKDFQELSLNEDAYMGVVDE ATLQKGSWSLLKDLKRITDS DVKGDLFVKELFWMLHLQKE TFATYVARFQKEAYCPNKCG VMLQTLIWCKNCKKEVHACR KSYDCGERNVEVPQMEDMIL DCELNWHQASEGLTDYSFYR VWGNNTETLVSKGKEATLTK PMVGPEDAGSYRCELGSVN |
| Tissue Specificity | Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction. |
| Post Translational Modifications | N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm. Phosphorylated. The cytoplasmic C-terminus is phosphorylated and undergoes phosphorylation changes during epididymal transit. |
| Function | Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization. FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion. |
| Protein Name | Izumo Sperm-Egg Fusion Protein 1Oocyte Binding/Fusion FactorObfSperm-Specific Protein Izumo |
| Database Links | Reactome: R-HSA-1300645 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmic VesicleSecretory VesicleAcrosome MembraneLocalizes Initially To The Acrosome Membrane Of The Sperm Head (Both Outer And Inner Acrosomal Membranes)During The Acrosome ReactionTranslocates To The Plasma Membrane |
| Alternative Antibody Names | Anti-Izumo Sperm-Egg Fusion Protein 1 antibodyAnti-Oocyte Binding/Fusion Factor antibodyAnti-Obf antibodyAnti-Sperm-Specific Protein Izumo antibodyAnti-IZUMO1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
