| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ITSN1 (750-850) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ITSN1 |
| Gene ID: |
6453 |
| Uniprot ID: |
ITSN1_HUMAN |
| Immunogen Region: |
750-850 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human ITSN1 (NP_001317941.1). |
| Immunogen Sequence: |
KTGWFPANYAEKIPENEVPA PVKPVTDSTSAPAPKLALRE TPAPLAVTSSEPSTTPNNWA DFSSTWPTSTNEKPETDNWD AWAAQPSLTVPSAGQLRQRS A |
| Tissue Specificity | Isoform 1 is expressed almost exclusively in the brain. Isoform 2 is detected in brain, spleen, lung, liver, heart, skeletal muscle and kidney. Isoform 5 is primarily expressed in brain, spleen, lung and kidney (at protein level). Isoform 1 and isoform 2 are detected in brain. Isoform 2 is ubiquitous in adult and fetal tissues with high expression in skeletal muscle, heart, spleen, ovary, testis and all fetal tissues tested and low expression in thymus, blood, lung, liver and pancreas. Isoform 1 is expressed almost exclusively in the brain, in all brain regions. Not expressed in the spinal cord. |
| Function | Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex. Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits. Involved in endocytosis of activated EGFR, and probably also other growth factor receptors. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR).internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones. Inhibits ARHGAP31 activity toward RAC1. Isoform 1: Plays a role in synaptic vesicle endocytosis in brain neurons. |
| Protein Name | Intersectin-1Sh3 Domain-Containing Protein 1aSh3p17 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-3928662Reactome: R-HSA-416482Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9013148Reactome: R-HSA-9013406Reactome: R-HSA-9013408 |
| Cellular Localisation | Endomembrane SystemSynapseSynaptosomeCell ProjectionLamellipodiumCell MembraneMembraneClathrin-Coated PitRecycling EndosomeEndosomeCytoplasmic VesicleColocalizes With Sgip1 At The Plasma Membrane In Structures Corresponding Most Probably To Clathrin-Coated PitsColocalizes With Rab13 On Cytoplasmic Vesicles That Are Most Likely Recycling EndosomesIsoform 2: CytoplasmNucleus EnvelopeShuttles Between The Cytoplasm And Nucleus In An Xpo1/Crm1-Dependent MannerIsoform 5: Endomembrane System |
| Alternative Antibody Names | Anti-Intersectin-1 antibodyAnti-Sh3 Domain-Containing Protein 1a antibodyAnti-Sh3p17 antibodyAnti-ITSN1 antibodyAnti-ITSN antibodyAnti-SH3D1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
