• Immunofluorescence analysis of L929 cells using INMT Rabbit polyclonal antibody (STJ116603) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using INMT Rabbit polyclonal antibody (STJ116603) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using INMT Rabbit polyclonal antibody (STJ116603) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-INMT antibody (1-263) (STJ116603)

SKU:
STJ116603

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INMT (1-263) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INMT
Gene ID: 11185
Uniprot ID: INMT_HUMAN
Immunogen Region: 1-263
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human INMT (NP_006765.4).
Immunogen Sequence: MKGGFTGGDEYQKHFLPRDY LATYYSFDGSPSPEAEMLKF NLECLHKTFGPGGLQGDTLI DIGSGPTIYQVLAACDSFQD ITLSDFTDRNREELEKWLKK EPGAYDWTPAVKFACELEGN SGRWEEKEEKLRAAVKRVLK CDVHLGNPLAPAVLPLADCV LTLLAMECACCSLDAYRAAL CNLASLLKPGGHLVTTVTLR LPSYMVGKREFSCVALEKEE VEQAVLDAGFDIEQLLHSP
Tissue Specificity Widely expressed. The highest levels were in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in adult and fetal kidney and liver, in adult spleen, thymus, ovary, colon and bone marrow. Not expressed in peripheral blood leukocytes and brain.
Function Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds. Catalyzes the N-methylation of tryptamine and structurally related compounds.
Protein Name Indolethylamine N-Methyltransferase
Indolamine N-Methyltransferase
Aromatic Alkylamine N-Methyltransferase
Amine N-Methyltransferase
Arylamine N-Methyltransferase
Thioether S-Methyltransferase
Temt
Database Links Reactome: R-HSA-2408552
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Indolethylamine N-Methyltransferase antibody
Anti-Indolamine N-Methyltransferase antibody
Anti-Aromatic Alkylamine N-Methyltransferase antibody
Anti-Amine N-Methyltransferase antibody
Anti-Arylamine N-Methyltransferase antibody
Anti-Thioether S-Methyltransferase antibody
Anti-Temt antibody
Anti-INMT antibody

Information sourced from Uniprot.org

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