| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Insulin-like growth factor-binding protein 3 (151-200 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:100-500 IF ICC 1:100-500 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IGFBP3 |
| Gene ID: | 3486 |
| Uniprot ID: | IBP3_HUMAN |
| Immunogen Region: | 151-200 aa |
| Specificity: | IGFBP3 Polyclonal Antibody detects endogenous levels of IGFBP3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human IGFBP-3 at the amino acid range 151-200 |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 3 Ibp-3 Igf-Binding Protein 3 Igfbp-3 |
| Database Links | Reactome: R-HSA-381426 Reactome: R-HSA-6803211 Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted Nucleus |
| Alternative Antibody Names | Anti-Insulin-Like Growth Factor-Binding Protein 3 antibody Anti-Ibp-3 antibody Anti-Igf-Binding Protein 3 antibody Anti-Igfbp-3 antibody Anti-IGFBP3 antibody Anti-IBP3 antibody |
Information sourced from Uniprot.org