• Immunohistochemistry analysis of IBA-1. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of IBA-1. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry of paraffin embedded rat brain using IBA-1 at dilution of 1:2000
  • Immunofluorescent analysis of paraffin embedded rat hippocampus using Neun + Iba1 + MBP + GFAP and Dapi in blue at dilution of 1:1000
  • Immunofluorescence of paraffin embedded mouse brain using IBA-1 at dilution of 1:1000
  • Immunofluorescent analysis of paraffin embedded rat brain using Neun+ Iba1 + MAP2 and Dapi in blue at dilution of 1:1000
  • IF analysis of IBA-1. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of IBA-1. Sample: Human glioblastoma multiforme, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of IBA-1. Sample: Human oligodendrocytoma, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of IBA-1. Sample: Monkey brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.

Anti-Iba1 antibody [SM4917] (STJA0004917)

SKU:
STJA0004917

£170.50 - £233.50
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Host: Mouse
Applications: IHC-P/IF-P
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Iba1 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM4917
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: IHC (PFA fixed) /IF (PFA fixed) (H, M, R, Mk) 1:500-1:4000/1:500-1:2000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Aif1
Gene ID: 11629
Uniprot ID: AIF1_MOUSE
Immunogen: Recombinant protein corresponding to Mouse Iba1
Post Translational Modifications Phosphorylated on serine residues.
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Protein Name Allograft Inflammatory Factor 1
Aif-1
Ionized Calcium-Binding Adapter Molecule 1
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Phagocytic Cup
Associated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis
Alternative Antibody Names Anti-Allograft Inflammatory Factor 1 antibody
Anti-Aif-1 antibody
Anti-Ionized Calcium-Binding Adapter Molecule 1 antibody
Anti-Aif1 antibody
Anti-Iba1 antibody

Information sourced from Uniprot.org

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