• Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2, 000.
  • IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
  • Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Mouse mAb diluted at 1:500.
  • Immunofluorescence analysis of Rat-testis tissue. 1, HSP70 monoclonal antibody (3G10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:2000.
  • Immunofluorescence analysis of Human-breast-cancer tissue. 1, HSP70 monoclonal antibody (3G10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Mouse-lung tissue. 1, HSP70 monoclonal antibody (3G10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, HSP70 monoclonal antibody (3G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, HSP70 monoclonal antibody (3G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, HSP70 monoclonal antibody (3G10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.
  • Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP70 antibody [3G10] (STJ96944)

SKU:
STJ96944

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Heat shock 70 kDa protein 1-like and OBSOLETE is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 3G10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000-2000
IF 1:100-200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA1L
Gene ID: 3305
Uniprot ID: HS71L_HUMAN
Specificity: The antibody detects endogenous HSP70 proteins.
Immunogen: Synthetic Peptide of HSP70
Function Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Positive regulator of PRKN translocation to damaged mitochondria.
Protein Name Heat Shock 70 Kda Protein 1-Like
Heat Shock 70 Kda Protein 1l
Heat Shock 70 Kda Protein 1-Hom
Hsp70-Hom
Database Links Reactome: R-HSA-3371453
Reactome: R-HSA-3371497
Reactome: R-HSA-3371568
Reactome: R-HSA-3371571
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 1-Like antibody
Anti-Heat Shock 70 Kda Protein 1l antibody
Anti-Heat Shock 70 Kda Protein 1-Hom antibody
Anti-Hsp70-Hom antibody
Anti-HSPA1L antibody

Information sourced from Uniprot.org

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