Western blot analysis of lysates from mouse brain, using HSD3B7 Rabbit pAb (STJ11106400) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-HSD3B7 antibody (120-280 aa) (STJ11106400)

SKU:
STJ11106400
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Gene Symbol: HSD3B7
Gene ID: 80270
Uniprot ID: 3BHS7_HUMAN
Immunogen Region: 120-280 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human HSD3B7 (NP_079469.2).
Immunogen Sequence: GTRFLVYTSSMEVVGPNTKG HPFYRGNEDTPYEAVHRHPY PCSKALAEWLVLEANGRKVR GGLPLVTCALRPTGIYGEGH QIMRDFYRQGLRLGGWLFRA IPASVEHGRVYVGNVAWMHV LAARELEQRATLMGGQVYFC YDGSPYRSYEDFNMEFLGPC G
Function The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.
Protein Name 3 Beta-Hydroxysteroid Dehydrogenase Type 7
3 Beta-Hydroxysteroid Dehydrogenase Type Vii
3-Beta-Hsd Vii
3-Beta-Hydroxy-Delta(5-C27 Steroid Oxidoreductase
C(27 3-Beta-Hsd
Cholest-5-Ene-3-Beta -7-Alpha-Diol 3-Beta-Dehydrogenase
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-3 Beta-Hydroxysteroid Dehydrogenase Type 7 antibody
Anti-3 Beta-Hydroxysteroid Dehydrogenase Type Vii antibody
Anti-3-Beta-Hsd Vii antibody
Anti-3-Beta-Hydroxy-Delta(5-C27 Steroid Oxidoreductase antibody
Anti-C(27 3-Beta-Hsd antibody
Anti-Cholest-5-Ene-3-Beta -7-Alpha-Diol 3-Beta-Dehydrogenase antibody
Anti-HSD3B7 antibody

Information sourced from Uniprot.org

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