• Immunofluorescence analysis of U-2 OS cells using hnRNP C Rabbit polyclonal antibody (STJ11100595) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of hnRNP C in paraffin-embedded mouse spinal cord using hnRNP C Rabbit polyclonal antibody (STJ11100595) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of hnRNP C in paraffin-embedded human brain using hnRNP C Rabbit polyclonal antibody (STJ11100595) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat testis, using hnRNP C Rabbit polyclonal antibody (STJ11100595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using hnRNP C Rabbit polyclonal antibody (STJ11100595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HNRNPC antibody (86-190) (STJ11100595)

SKU:
STJ11100595

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRNPC (86-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPC
Gene ID: 3183
Uniprot ID: HNRPC_HUMAN
Immunogen Region: 86-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 86-190 of human hnRNP C (NP_112604.2).
Immunogen Sequence: AEPKVNRGKAGVKRSAAEMY GSVTEHPSPSPLLSSSFDLD YDFQRDYYDRMYSYPARVPP PPPIARAVVPSKRQRVSGNT SRRGKSGFNSKSGQRGSSKS GKLKG
Post Translational Modifications Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide. Sumoylated. Sumoylation reduces affinity for mRNA.
Function Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.
Protein Name Heterogeneous Nuclear Ribonucleoproteins C1/C2
Hnrnp C1/C2
Database Links Reactome: R-HSA-4570464
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-9013418
Reactome: R-HSA-9013422
Cellular Localisation Nucleus
Component Of Ribonucleosomes
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoproteins C1/C2 antibody
Anti-Hnrnp C1/C2 antibody
Anti-HNRNPC antibody
Anti-HNRPC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance