| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HINT1 |
| Gene ID: | 3094 |
| Uniprot ID: | HINT1_HUMAN |
| Immunogen Region: | 1-126 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1). |
| Immunogen Sequence: | MADEIAKAQVARPGGDTIFG KIIRKEIPAKIIFEDDRCLA FHDISPQAPTHFLVIPKKHI SQISVAEDDDESLLGHLMIV GKKCAADLGLNKGYRMVVNE GSDGGQSVYHVHLHVLGGRQ MHWPPG |
| Tissue Specificity | Widely expressed. |
| Function | Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17. Deconjugates SUMO1 from RANGAP1. |
| Protein Name | Adenosine 5'-Monophosphoramidase Hint1 Desumoylating Isopeptidase Hint1 Histidine Triad Nucleotide-Binding Protein 1 Protein Kinase C Inhibitor 1 Protein Kinase C-Interacting Protein 1 Pkci-1 |
| Database Links | Reactome: R-HSA-9824594 Reactome: R-HSA-9825892 Reactome: R-HSA-9856649 |
| Cellular Localisation | Cytoplasm Nucleus Interaction With Cdk7 Leads To A More Nuclear Localization |
| Alternative Antibody Names | Anti-Adenosine 5'-Monophosphoramidase Hint1 antibody Anti-Desumoylating Isopeptidase Hint1 antibody Anti-Histidine Triad Nucleotide-Binding Protein 1 antibody Anti-Protein Kinase C Inhibitor 1 antibody Anti-Protein Kinase C-Interacting Protein 1 antibody Anti-Pkci-1 antibody Anti-HINT1 antibody Anti-HINT antibody Anti-PKCI1 antibody Anti-PRKCNH1 antibody |
Information sourced from Uniprot.org