• Western blot analysis of extracts of mouse kidney, using HEMK1 antibody (STJ114525) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HEMK1 antibody (1-338) (STJ114525)

SKU:
STJ114525

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:3000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HEMK1
Gene ID: 51409
Uniprot ID: HEMK1_HUMAN
Immunogen Region: 1-338
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human HEMK1 (NP_057257.1).
Immunogen Sequence: MELWGRMLWALLSGPGRRGS TRGWAFSSWQPQPPLAGLSS AIELVSHWTGVFEKRGIPEA RESSEYIVAHVLGAKTFQSL RPALWTQPLTSQQLQCIREL SSRRLQRMPVQYILGEWDFQ GLSLRMVPPVFIPRPETEEL VEWVLEEVAQRSHAVGSPGS PLILEVGCGSGAISLSLLSQ LPQSRVIAVDKREAAISLTH ENAQRLRLQDRIWIIHLDMT SERSWTHLPWGPMDLIVSN
Function N5-glutamine methyltransferase responsible for the methylation of the glutamine residue in the universally conserved GGQ motif of the mitochondrial translation release factors MTRF1, MTRF1L, MRPL58/ICT1 and MTRFR.
Protein Name Mtrf1l Release Factor Glutamine Methyltransferase
Hemk Methyltransferase Family Member 1
M.hsahemkp
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Mtrf1l Release Factor Glutamine Methyltransferase antibody
Anti-Hemk Methyltransferase Family Member 1 antibody
Anti-M.hsahemkp antibody
Anti-HEMK1 antibody
Anti-HEMK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance