• Western blot analysis of extracts from wild type (WT) and HDAC10 knockdown (KD) 293T (KD) cells, using HDAC10 Rabbit polyclonal antibody (STJ11105309) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of MCF7, using HDAC10 Rabbit polyclonal antibody (STJ11105309) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HDAC10 antibody (1-100 aa) (STJ11105309)

SKU:
STJ11105309

£129.50 - £642.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC10
Gene ID: 83933
Uniprot ID: HDA10_HUMAN
Immunogen Region: 1-100 aa
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC10. (NP_114408.3).
Immunogen Sequence: MGTALVYHEDMTATRLLWDD PECEIERPERLTAALDRLRQ RGLEQRCLRLSAREASEEEL GLVHSPEYVSLVRETQVLGK EELQALSGQFDAIYFHPSTF
Tissue Specificity Widely expressed with high levels in liver and kidney.
Function Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak. May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells. May play a role in homologous recombination. May promote DNA mismatch repair.
Protein Name Polyamine Deacetylase Hdac10
Histone Deacetylase 10
Hd10
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Cellular Localisation Cytoplasm
Nucleus
Excluded From Nucleoli
Alternative Antibody Names Anti-Polyamine Deacetylase Hdac10 antibody
Anti-Histone Deacetylase 10 antibody
Anti-Hd10 antibody
Anti-HDAC10 antibody

Information sourced from Uniprot.org

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