-
Western blot analysis of 1) Hela, 2) 3T3, 3) Raw264.7, 4) Rat Brain, 5) Rat Kidney, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
-
Immunofluorescence analysis of Rat-heart tissue. 1, Histone H2B monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
-
Immunofluorescence analysis of Human-liver-cancer tissue. 1, Histone H2B monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
-
Immunofluorescence analysis of Mouse-kidney tissue. 1, Histone H2B monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
-
Immunofluorescence analysis of Hela cell. 1, Brp44L Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
