| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GRK4 |
| Gene ID: | 2868 |
| Uniprot ID: | GRK4_HUMAN |
| Immunogen Region: | 359-578 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 359-578 of human GRK4 (NP_892027.2). |
| Immunogen Sequence: | NNEKYTFSPDWWGLGCLIYE MIQGHSPFKKYKEKVKWEEV DQRIKNDTEEYSEKFSEDAK SICRMLLTKNPSKRLGCRGE GAAGVKQHPVFKDINFRRLE ANMLEPPFCPDPHAVYCKDV LDIEQFSVVKGIYLDTADED FYARFATGCVSIPWQNEMIE SGCFKDINKSESEEALPLDL DKNIHTPVSRPNRGFFYRLF RRGGCLTMVPSEKEVEPKQC |
| Tissue Specificity | Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium. |
| Post Translational Modifications | Palmitoylated. |
| Function | Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin.the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. |
| Protein Name | G Protein-Coupled Receptor Kinase 4 G Protein-Coupled Receptor Kinase Grk4 Iti1 |
| Database Links | Reactome: R-HSA-2514859 P32298-1 |
| Cellular Localisation | Cytoplasm Cell Cortex Both At The Cell Surface And Dispersed In The Cytoplasm Under Basal Conditions Receptor Stimulation Results In The Internalization Of Grk4 To The Perinuclear Area Where Colocalization With Drd3 Is Observed Strongly At 5 And 15 Minutes Drd3 And Grk4 Colocalize In Lipid Rafts Of Renal Proximal Tubule Cells |
| Alternative Antibody Names | Anti-G Protein-Coupled Receptor Kinase 4 antibody Anti-G Protein-Coupled Receptor Kinase Grk4 antibody Anti-Iti1 antibody Anti-GRK4 antibody Anti-GPRK2L antibody Anti-GPRK4 antibody |
Information sourced from Uniprot.org