• Immunofluorescence analysis of L929 cells using GPRASP1 Rabbit polyclonal antibody (STJ118530) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GPRASP1 Rabbit polyclonal antibody (STJ118530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPRASP1 antibody (200-500) (STJ118530)

SKU:
STJ118530

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPRASP1 (200-500) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPRASP1
Gene ID: 9737
Uniprot ID: GASP1_HUMAN
Immunogen Region: 200-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human GPRASP1 (NP_055525.3).
Immunogen Sequence: SSLFWSGDEVTAKFHPGNRV KDSNRSMHMANQEANTMSRS QTNQELYIASSSGSEDESVK TPWFWARDKTNTWSGPREDP NSRSRFRSKKEVYVESSSGS EHEDHLESWFGAGKEAKFRS KMRAGKEANNRARHRAKREA CIDFMPGSIDVIKKESCFWP EENANTFSRPMIKKEARARA MTKEEAKTKARARAKQEARS EEEALIGTWFWATDESSMAD EASIESSLQVEDESIIGSW
Tissue Specificity Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra.
Function Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.
Protein Name G-Protein Coupled Receptor-Associated Sorting Protein 1
Gasp-1
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-G-Protein Coupled Receptor-Associated Sorting Protein 1 antibody
Anti-Gasp-1 antibody
Anti-GPRASP1 antibody
Anti-GASP antibody
Anti-KIAA0443 antibody

Information sourced from Uniprot.org

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