• Western blot analysis of extracts of various cell lines, using GPC5 antibody (STJ112446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPC5 antibody (320-550) (STJ112446)

SKU:
STJ112446

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPC5
Gene ID: 2262
Uniprot ID: GPC5_HUMAN
Immunogen Region: 320-550
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 320-550 of human GPC5 (NP_004457.1).
Immunogen Sequence: HLLVNDAVLQAHLNGQKLLE QVNRICGRPVRTPTQSPRCS FDQSKEKHGMKTTTRNSEET LANRRKEFINSLRLYRSFYG GLADQLCANELAAADGLPCW NGEDIVKSYTQRVVGNGIKA QSGNPEVKVKGIDPVINQII DKLKHVVQLLQGRSPKPDKW ELLQLGSGGGMVEQVSGDCD DEDGCGGSGSGEVKRTLKIT DWMPDDMNFSDVKQIHQTDT GSTLDTTGAGC
Tissue Specificity In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.
Function Cell surface proteoglycan that bears heparan sulfate.
Protein Name Glypican-5 Cleaved Into - Secreted Glypican-5
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-4420332
Reactome: R-HSA-5362798
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Reactome: R-HSA-9820960
Reactome: R-HSA-9833110
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Secreted Glypican-5: Secreted
Extracellular Space
Alternative Antibody Names Anti-Glypican-5 Cleaved Into - Secreted Glypican-5 antibody
Anti-GPC5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance