| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GPBAR1 (223-322) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GPBAR1 |
| Gene ID: |
151306 |
| Uniprot ID: |
GPBAR_HUMAN |
| Immunogen Region: |
223-322 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 223-322 of human GPBAR1 (NP_733800.1). |
| Immunogen Sequence: |
ARAQAGAMLLFGLCWGPYVA TLLLSVLAYEQRPPLGPGTL LSLLSLGSASAAAVPVAMGL GDQRYTAPWRAAAQRCLQGL WGRASRDSPGPSIAYHPSSQ |
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum. |
| Function | Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids. |
| Protein Name | G-Protein Coupled Bile Acid Receptor 1G-Protein Coupled Receptor Gpcr19Hgpcr19Membrane-Type Receptor For Bile AcidsM-BarHbg37Bg37 |
| Database Links | Reactome: R-HSA-373076Reactome: R-HSA-418555 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Protein Coupled Bile Acid Receptor 1 antibodyAnti-G-Protein Coupled Receptor Gpcr19 antibodyAnti-Hgpcr19 antibodyAnti-Membrane-Type Receptor For Bile Acids antibodyAnti-M-Bar antibodyAnti-Hbg37 antibodyAnti-Bg37 antibodyAnti-GPBAR1 antibodyAnti-TGR5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89668/358164/STJ11100730_1__42931.1713122467.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
