• Western blot analysis of extracts of various cell lines, using GNPAT antibody (STJ23825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GNPAT antibody (481-680) (STJ23825)

SKU:
STJ23825

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNPAT
Gene ID: 8443
Uniprot ID: GNPAT_HUMAN
Immunogen Region: 481-680
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 481-680 of human GNPAT (NP_055051.1).
Immunogen Sequence: YRNQLLNIFVRPSLVAVALQ MTPGFRKEDVYSCFRFLRDV FADEFIFLPGNTLKDFEEGC YLLCKSEAIQVTTKDILVTE KGNTVLEFLVGLFKPFVESY QIICKYLLSEEEDHFSEEQY LAAVRKFTSQLLDQGTSQCY DVLSSDVQKNALAACVRLGV VEKKKINNNCIFNVNEPATT KLEEMLGCKTPIGKPATAKL
Function Dihydroxyacetonephosphate acyltransferase catalyzing the first step in the biosynthesis of plasmalogens, a subset of phospholipids that differ from other glycerolipids by having an alkyl chain attached through a vinyl ether linkage at the sn-1 position of the glycerol backbone, and which unique physical properties have an impact on various aspects of cell signaling and membrane biology.
Protein Name Dihydroxyacetone Phosphate Acyltransferase
Dap-At
Dapat
Dhap-At
Acyl-Coa -Dihydroxyacetonephosphateacyltransferase
Glycerone-Phosphate O-Acyltransferase
Database Links Reactome: R-HSA-1483166
Reactome: R-HSA-75896
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome Membrane
Peripheral Membrane Protein
Matrix Side
Exclusively Localized To The Lumenal Side Of The Peroxisomal Membrane
Alternative Antibody Names Anti-Dihydroxyacetone Phosphate Acyltransferase antibody
Anti-Dap-At antibody
Anti-Dapat antibody
Anti-Dhap-At antibody
Anti-Acyl-Coa -Dihydroxyacetonephosphateacyltransferase antibody
Anti-Glycerone-Phosphate O-Acyltransferase antibody
Anti-GNPAT antibody
Anti-DAPAT antibody
Anti-DHAPAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance