| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (592-641 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GNE |
| Gene ID: | 10020 |
| Uniprot ID: | GLCNE_HUMAN |
| Immunogen Region: | 592-641 aa |
| Specificity: | GLCNE Polyclonal Antibody detects endogenous levels of GLCNE protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human GNE at the amino acid range 592-641 |
| Post Translational Modifications | Phosphorylated. Phosphorylation by PKC activates the UDP-N-acetylglucosamine 2-epimerase activity. |
| Function | Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis. Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition. |
| Protein Name | Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase Hydrolyzing Udp-Glcnac-2-Epimerase Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase Manac Kinase |
| Database Links | Reactome: R-HSA-4085001 Reactome: R-HSA-4085011 |
| Cellular Localisation | Cytoplasm Cytosol |
| Alternative Antibody Names | Anti-Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase antibody Anti-Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase antibody Anti-Hydrolyzing antibody Anti-Udp-Glcnac-2-Epimerase antibody Anti-Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase antibody Anti-Manac Kinase antibody Anti-GNE antibody Anti-GLCNE antibody Anti-IBM2 antibody |
Information sourced from Uniprot.org