• Immunohistochemistry analysis of Glutathione Peroxidase 4. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of Glutathione Peroxidase 4. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of Glutathione Peroxidase 4. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of Glutathione Peroxidase 4. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • Immunohistochemistry analysis of Glutathione Peroxidase 4. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Primary antibody: 1:500, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
  • IF analysis of Glutathione Peroxidase 4red. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.
  • IF analysis of Glutathione Peroxidase 4red. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:300, RT, 1h.

Anti-Glutathione Peroxidase 4 antibody [SM0379] (STJA0010379)

SKU:
STJA0010379

€213.13 - €291.88
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Host: Mouse
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Glutathione Peroxidase 4 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM0379
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (M, R) 1:500-1:1500
IHC/IF (M, R) 1:500-1:1500
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Gpx4
Gene ID: 625249
Uniprot ID: GPX4_MOUSE
Immunogen: Recombinant protein corresponding to Mouse GPX4
Function Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins. Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide. Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis. The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures. May be required to protect cells from the toxicity of ingested lipid hydroperoxides. Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion. Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism. Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway. Can also reduce small soluble hydroperoxides such as H2O2 and tert-butyl hydroperoxide. Isoform Cytoplasmic: Specifically able to suppress the production of leukotriene and prostaglandin in response to several stimuli by reducing fatty acid hydroperoxide. Isoform Mitochondrial: Specifically required to prevent mitochondrial cell death by mediating reduction of cardiolipin hydroperoxide. Also required for normal sperm development and male fertility. Isoform Nuclear: Required for male fertility by stabilizing the condensed chromatin in sperm nuclei.
Protein Name Phospholipid Hydroperoxide Glutathione Peroxidase Gpx4
Phgpx
Glutathione Peroxidase 4
Gpx-4
Gshpx-4
Database Links Reactome: R-MMU-2142712
Reactome: -MMU-2142770
Reactome: -MMU-9018676
Reactome: -MMU-9018896
Reactome: -MMU-9020265
Reactome: -MMU-9023661
Cellular Localisation Isoform Mitochondrial: Mitochondrion
Isoform Cytoplasmic: Cytoplasm
Isoform Nuclear: Nucleus
Alternative Antibody Names Anti-Phospholipid Hydroperoxide Glutathione Peroxidase Gpx4 antibody
Anti-Phgpx antibody
Anti-Glutathione Peroxidase 4 antibody
Anti-Gpx-4 antibody
Anti-Gshpx-4 antibody
Anti-Gpx4 antibody

Information sourced from Uniprot.org

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