• Western blot analysis of extracts of various cell lines, using GDF10 antibody (STJ112577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GDF10 antibody (369-478) (STJ112577)

SKU:
STJ112577

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GDF10
Gene ID: 2662
Uniprot ID: GDF10_HUMAN
Immunogen Region: 369-478
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 369-478 of human GDF10 (NP_004953.1).
Immunogen Sequence: QWDEPRVCSRRYLKVDFADI GWNEWIISPKSFDAYYCAGA CEFPMPKIVRPSNHATIQSI VRAVGIIPGIPEPCCVPDKM NSLGVLFLDENRNVVLKVYP NMSVDTCACR
Tissue Specificity Expressed in femur, brain, lung, skeletal muscle, pancreas and testis.
Function Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis.
Protein Name Growth/Differentiation Factor 10
Gdf-10
Bone Morphogenetic Protein 3b
Bmp-3b
Bone-Inducing Protein
Bip
Cellular Localisation Secreted
Alternative Antibody Names Anti-Growth/Differentiation Factor 10 antibody
Anti-Gdf-10 antibody
Anti-Bone Morphogenetic Protein 3b antibody
Anti-Bmp-3b antibody
Anti-Bone-Inducing Protein antibody
Anti-Bip antibody
Anti-GDF10 antibody
Anti-BMP3B antibody

Information sourced from Uniprot.org