| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GABAA Receptor a 4 (Cytoplasmic Loop) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was antigen affinity purified from pooled serum. |
| Dilution Range: |
WB 1:1000IHC 1:300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Gabra4 |
| Gene ID: |
140675 |
| Uniprot ID: |
GBRA4_RAT |
| Immunogen Region: |
Cytoplasmic Loop |
| Immunogen: |
Fusion protein from the cytoplasmic loop of the alpha 4 subunit |
| Function | Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). Alpha-4/GABRA4 subunit often assembles with delta or gamma-2 subunits, in combination with beta subunits. When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-4 are predominantly extrasynaptic, contributing to tonic inhibition in dentate granule cells and thalamic relay neurons. Extrasynaptic alpha-4-containing GABAARs control levels of excitability and network activity. GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness. |
| Protein Name | Gamma-Aminobutyric Acid Receptor Subunit Alpha-4Gaba(A Receptor Subunit Alpha-4Gabaar Subunit Alpha-4 |
| Database Links | Reactome: R-RNO-977443 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPostsynaptic Cell Membrane |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-4 antibodyAnti-Gaba(A Receptor Subunit Alpha-4 antibodyAnti-Gabaar Subunit Alpha-4 antibodyAnti-Gabra4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
