| Host: | Rabbit |
| Applications: | IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Frizzled-4 (131-180 aa) is suitable for use in Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IF 1:200-1:1000 ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FZD4 |
| Gene ID: | 8322 |
| Uniprot ID: | FZD4_HUMAN |
| Immunogen Region: | 131-180 aa |
| Specificity: | Frizzled-4 Polyclonal Antibody detects endogenous levels of Frizzled-4 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human FZD4 at the amino acid range 131-180 |
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
| Protein Name | Frizzled-4 Fz-4 Hfz4 Fze4 Cd Antigen Cd344 |
| Database Links | Reactome: R-HSA-373080 Reactome: R-HSA-4086398 Reactome: R-HSA-4608870 Reactome: R-HSA-4641263 Reactome: R-HSA-5099900 Reactome: R-HSA-5340588 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Frizzled-4 antibody Anti-Fz-4 antibody Anti-Hfz4 antibody Anti-Fze4 antibody Anti-Cd Antigen Cd344 antibody Anti-FZD4 antibody |
Information sourced from Uniprot.org