• STJ71475 (0. 03µg/ml) staining of Human Heart (A) and (0. 1ug/ml) Placenta (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71475 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Frizzled 2 antibody (Internal) (STJ71475)

SKU:
STJ71475-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Frizzled 2 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2-5µg/ml, 2.5µg/ml
IF-Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: FZD2
Gene ID: 2535
Uniprot ID: FZD2_HUMAN
Immunogen Region: Internal
Accession Number: NP_001457.1
Immunogen Sequence: RYATLEHPFHCPR
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling.
Protein Name Frizzled-2
Fz-2
Hfz2
Fze2
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-373080
Reactome: R-HSA-4086398
Reactome: R-HSA-4608870
Reactome: R-HSA-4641262
Reactome: R-HSA-5140745
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Frizzled-2 antibody
Anti-Fz-2 antibody
Anti-Hfz2 antibody
Anti-Fze2 antibody
Anti-FZD2 antibody

Information sourced from Uniprot.org

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