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Anti-FN1 antibody [M9] (STJ97039)

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Western blot analysis of Hela, diluted at 1:2000.
Immunofluorescence analysis of Mouse-spleen tissue. 1, Fibronectin monoclonal antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-appendix tissue. 1, Fibronectin monoclonal antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Fibronectin monoclonal antibody (M9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Fibronectin monoclonal antibody (M9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-FN1 antibody [M9] (STJ97039)

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STJ97039

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Fibronectin is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	M9
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range:	WB 1:1000-2000 IF 1:200 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	FN1
Gene ID:	2335
Uniprot ID:	FINC_HUMAN
Specificity:	The antibody detects endogenous Fibronectin protein.
Immunogen:	Synthetic Peptide of Fibronectin

Post Translational Modifications	Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia.
Function	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Protein Name	Fibronectin Fn Cold-Insoluble Globulin Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1474228 Reactome: R-HSA-1474244 Reactome: R-HSA-1566977 Reactome: R-HSA-202733 Reactome: R-HSA-2129379 Reactome: R-HSA-216083 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-354192 Reactome: R-HSA-354194 Reactome: R-HSA-372708 Reactome: R-HSA-381426 Reactome: R-HSA-5674135 Reactome: R-HSA-6785807 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-8874081 Reactome: R-HSA-8957275 Reactome: R-HSA-9634597 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9700645 Reactome: R-HSA-9725370 Reactome: R-HSA-9725371
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Fibronectin antibody Anti-Fn antibody Anti-Cold-Insoluble Globulin antibody Anti-Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 antibody Anti-FN1 antibody Anti-FN antibody

Information sourced from Uniprot.org

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