| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FEM1A (100-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FEM1A |
| Gene ID: |
55527 |
| Uniprot ID: |
FEM1A_HUMAN |
| Immunogen Region: |
100-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human FEM1A (NP_061178.1). |
| Immunogen Sequence: |
RSLLRRGASVNRTTRTNSTP LRAACFDGHLEVVRYLVGEH QADLEVANRHGHTCLMISCY KGHREIARYLLEQGAQVNRR SAKGNTALHDCAESGSLEIL QLLLGCKARMERDGYGMTPL LAASVTGHTNIVEYLIQEQP GQEQVAGGEAQPGLPQEDPS TSQGCAQPQGAPCCSSSPEE PLNGESYESCCPTSREAAVE A |
| Tissue Specificity | Present in macrophages derived from peripheral blood monocytes. Also present in atheromata (at protein level). |
| Post Translational Modifications | Phosphorylated.highly phosphorylated in myoblasts and myotubes. Phosphorylation at Ser-108 promotes PGE2-EP4-mediated inhibition of inflammation. Dephosphorylated by protein phosphatase 2A (PP2A). |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(FEM1A) complex specifically recognizes proteins with an arginine at the C-terminus: recognizes and binds proteins ending with -Lys/Arg-Xaa-Arg and -Lys/Arg-Xaa-Xaa-Arg C-degrons, such as SIL1 or OR51B2, leading to their ubiquitination and degradation. Promotes ubiquitination and degradation of SLBP. Involved in PGE2-EP4-mediated inhibition of inflammation of macrophages via interaction with NFKB1 and PTGER4. Promotes inflammation in brain microglia through MAP2K4/MKK4-mediated signaling. |
| Protein Name | Protein Fem-1 Homolog AFem1aFem1-AlphaProstaglandin E Receptor 4-Associated Protein |
| Database Links | Reactome: R-HSA-8951664 |
| Cellular Localisation | MitochondrionCytoplasm |
| Alternative Antibody Names | Anti-Protein Fem-1 Homolog A antibodyAnti-Fem1a antibodyAnti-Fem1-Alpha antibodyAnti-Prostaglandin E Receptor 4-Associated Protein antibodyAnti-FEM1A antibodyAnti-EPRAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
