Western blot analysis of lysates from RAW264. 7 cells, using EZH1 Rabbit polyclonal antibody (STJ117467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EZH1 antibody (160-270) (STJ117467)

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STJ117467
€140.63 - €655.63
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EZH1
Gene ID: 2145
Uniprot ID: EZH1_HUMAN
Immunogen Region: 160-270
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 160-270 of EZH1 (NP_001982.2).
Immunogen Sequence: GEEEMIPGSVLISDAVFLEL VDALNQYSDEEEEGHNDTSD GKQDDSKEDLPVTRKRKRHA IEGNKKSSKKQFPNDMIFSA IASMFPENGVPDDMKERYRE LTEMSDPNALP
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Protein Name Histone-Lysine N-Methyltransferase Ezh1
Enx-2
Enhancer Of Zeste Homolog 1
Cellular Localisation Nucleus
Colocalizes With Trimethylated 'Lys-27' Of Histone H3
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Ezh1 antibody
Anti-Enx-2 antibody
Anti-Enhancer Of Zeste Homolog 1 antibody
Anti-EZH1 antibody
Anti-KIAA0388 antibody

Information sourced from Uniprot.org

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