Anti-EXOSC10 antibody (586-885) (STJ119611)

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STJ119611
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXOSC10
Gene ID: 5394
Uniprot ID: EXOSX_HUMAN
Immunogen Region: 586-885
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 586-885 of human EXOSC10 (NP_001001998.1).
Immunogen Sequence: VAAGVKKSGPLPSAERLENV LFGPHDCSHAPPDGYPIIPT SGSVPVQKQASLFPDEKEDN LLGTTCLIATAVITLFNEPS AEDSKKGPLTVAQKKAQNIM ESFENPFRMFLPSLGHRAPV SQAAKFDPSTKIYEISNRWK LAQVQVQKDSKEAVKKKAAE QTAAREQAKEACKAAAEQAI SVRQQVVLENAAKKRERATS DPRTTEQKQEKKRLKISKKP KDPEPPEKEFTPYDYSQSD
Post Translational Modifications Sumoylated by USP36.sumoylation does not significantly affect EXOSC10 nucleolar localization and association with core exosome and USP36, but regulates the nucleolar RNA exosome activity in rRNA processing by promoting binding of EXOSC10 to pre-rRNAs. Effects of sumoylation on EXOSC10 levels vary between different studies. Sumoylation of EXOSC10 is required for the modulation of EXOSC10 effects on cellular protein translation and cell proliferation. Sumoylation is promoted by mild hypothermia.
Function Catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPHOSPH6 with the RNA exosome involved in the maturation of 5.8S rRNA. Plays a role in the recruitment of replication protein A complex (RPA) and RAD51 to DNA double-strand breaks caused by irradiation, contributing to DNA repair by homologous recombination. Regulates levels of damage-induced RNAs in order to prevent DNA-RNA hybrid formation at DNA double-strand breaks and limit DNA end resection after damage. Plays a role in oocyte development, maturation and survival. Required for normal testis development and mitotic division of spermatogonia. Plays a role in proper embryo development. Required for global protein translation. Required for cell proliferation. Regulates metabolism of C9orf72-derived repeat RNA that can be translated into toxic dipeptide repeat proteins.
Protein Name Exosome Complex Component 10
Autoantigen Pm/Scl 2
P100 Polymyositis-Scleroderma Overlap Syndrome-Associated Autoantigen
Polymyositis/Scleroderma Autoantigen 100 Kda
Pm/Scl-100
Polymyositis/Scleroderma Autoantigen 2
Database Links Reactome: R-HSA-6791226
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Nucleoplasm
Strongly Enriched In The Nucleolus And A Small Amount Has Been Found In Cytoplasm Supporting The Existence Of A Nucleolar Rna Exosome Complex Form
Arginine-Rich Dipeptide Repeat Proteins Expressed From C9orf72-Derived Repeat Rna Cause Diffuse Nuclear Misdistribution Of Exosc10
Relocates To The Dna Double-Strand Breaks In Response To Irradiation
Alternative Antibody Names Anti-Exosome Complex Component 10 antibody
Anti-Autoantigen Pm/Scl 2 antibody
Anti-P100 Polymyositis-Scleroderma Overlap Syndrome-Associated Autoantigen antibody
Anti-Polymyositis/Scleroderma Autoantigen 100 Kda antibody
Anti-Pm/Scl-100 antibody
Anti-Polymyositis/Scleroderma Autoantigen 2 antibody
Anti-EXOSC10 antibody
Anti-PMSCL antibody
Anti-PMSCL2 antibody
Anti-RRP6 antibody

Information sourced from Uniprot.org