| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ERVW-1 (480-538) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5 µg/µl |
| Dilution Range: | WB: 1:500 ELISA: 1:50, 000 IP: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | ERVW-1 |
| Gene ID: | 30816 |
| Uniprot ID: | SYCY1_HUMAN |
| Immunogen Region: | 480-538 |
| Immunogen: | Synthetic peptide taken within amino acid region 480-538 on human Syncytin-1 protein. |
| Tissue Specificity | Expressed at higher level in placental syncytiotrophoblast. Expressed at intermediate level in testis. Seems also to be found at low level in adrenal tissue, bone marrow, breast, colon, kidney, ovary, prostate, skin, spleen, thymus, thyroid, brain and trachea. Both mRNA and protein levels are significantly increased in the brain of individuals with multiple sclerosis, particularly in astrocytes and microglia. |
| Post Translational Modifications | Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as an inactive precursor that is heavily N-glycosylated and processed likely by furin in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence KR-X-KR-R. The intracytoplasmic tail cleavage by the viral protease that is required for the fusiogenic activity of some retroviruses envelope proteins seems to have been lost during evolution. The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion. |
| Function | This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein (SU) mediates receptor recognition, while the transmembrane protein (TM) acts as a class I viral fusion protein. The protein may have at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes. |
| Protein Name | Syncytin-1 Endogenous Retrovirus Group W Member 1 Env-W Envelope Polyprotein Gpr73 Enverin Herv-7q Envelope Protein Herv-W Envelope Protein Herv-W_7q21.2 Provirus Ancestral Env Polyprotein Syncytin Cleaved Into - Surface Protein Su Gp50 - Transmembrane Protein Tm Gp24 |
| Cellular Localisation | Surface Protein: Cell Membrane Peripheral Membrane Protein The Surface Protein Is Not Anchored To The Membrane But Localizes To The Extracellular Surface Through Its Binding To Tm Transmembrane Protein: Cell Membrane Single-Pass Type I Membrane Protein Syncytin-1: Virion |
| Alternative Antibody Names | Anti-Syncytin-1 antibody Anti-Endogenous Retrovirus Group W Member 1 antibody Anti-Env-W antibody Anti-Envelope Polyprotein Gpr73 antibody Anti-Enverin antibody Anti-Herv-7q Envelope Protein antibody Anti-Herv-W Envelope Protein antibody Anti-Herv-W_7q21.2 Provirus Ancestral Env Polyprotein antibody Anti-Syncytin Cleaved Into - Surface Protein antibody Anti-Su antibody Anti-Gp50 - Transmembrane Protein antibody Anti-Tm antibody Anti-Gp24 antibody Anti-ERVW-1 antibody Anti-ERVWE1 antibody |
Information sourced from Uniprot.org