| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Ephrin Receptor B3 (100-200) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.58-0.61 µg/µl |
| Dilution Range: | WB: 1:750 DB: 1:10, 000 ELISA: 1:10, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Ephb3 |
| Gene ID: | 13845 |
| Uniprot ID: | EPHB3_MOUSE |
| Immunogen Region: | 100-200 |
| Immunogen: | Synthetic peptide taken within amino acid region 100-200 on EphBR3 protein. |
| Tissue Specificity | Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level). Expressed in myogenic progenitor cells. |
| Post Translational Modifications | Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-609 is required for interaction with SH2 domain-containing proteins. Ubiquitinated by RNF186, mainly through 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. |
| Protein Name | Ephrin Type-B Receptor 3 Developmental Kinase 5 Mdk-5 Tyrosine-Protein Kinase Receptor Sek-4 |
| Database Links | Reactome: R-MMU-2682334 Reactome: -MMU-3928662 Reactome: -MMU-3928664 Reactome: -MMU-3928665 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Dendrite |
| Alternative Antibody Names | Anti-Ephrin Type-B Receptor 3 antibody Anti-Developmental Kinase 5 antibody Anti-Mdk-5 antibody Anti-Tyrosine-Protein Kinase Receptor Sek-4 antibody Anti-Ephb3 antibody Anti-Etk2 antibody Anti-Mdk5 antibody Anti-Sek4 antibody |
Information sourced from Uniprot.org