| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EML3 |
| Gene ID: | 256364 |
| Uniprot ID: | EMAL3_HUMAN |
| Immunogen Region: | 300-530 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-530 of human EML3 (NP_694997.2). |
| Immunogen Sequence: | HYRGHTDCVRCLAVHPDGVR VASGQTAGVDKDGKPLQPVV HIWDSETLLKLQEIGLGAFE RGVGALAFSAADQGAFLCVV DDSNEHMLSVWDCSRGMKLA EIKSTNDSVLAVGFNPRDSS CIVTSGKSHVHFWNWSGGVG VPGNGTLTRKQGVFGKYKKP KFIPCFVFLPDGDILTGDSE GNILTWGRSPSDSKTPGRGG AKETYGIVAQAHAHEGSIFA LCLRRDGTVLS |
| Post Translational Modifications | Phosphorylation at Thr-881 during mitosis is required for interaction with TUBG1, HAUS1, HAUS2, HAUS3, HAUS4, HAUS5, HAUS6, HAUS7 and HAUS8 and their recruitment to spindle microtubules. |
| Function | Regulates mitotic spindle assembly, microtubule (MT)-kinetochore attachment and chromosome separation via recruitment of HAUS augmin-like complex and TUBG1 to the existing MTs and promoting MT-based MT nucleation. Required for proper alignnment of chromosomes during metaphase. |
| Protein Name | Echinoderm Microtubule-Associated Protein-Like 3Emap-3 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusMidbodySpindleLocalizes To Microtubules Throughout All Mitotic Stages And Localizes To The Midbody During Cytokinesis |
| Alternative Antibody Names | Anti-Echinoderm Microtubule-Associated Protein-Like 3 antibodyAnti-Emap-3 antibodyAnti-EML3 antibody |
Information sourced from Uniprot.org