• Western blot analysis of extracts of various cell lines, using EIF4H antibody (STJ111994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using EIF4H antibody (STJ111994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF4H antibody (1-228) (STJ111994)

SKU:
STJ111994

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF4H (1-228) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4H
Gene ID: 7458
Uniprot ID: IF4H_HUMAN
Immunogen Region: 1-228
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human EIF4H (NP_114381.1).
Immunogen Sequence: MADFDTYDDRAYSSFGGGRG SRGSAGGHGSRSQKELPTEP PYTAYVGNLPFNTVQGDIDA IFKDLSIRSVRLVRDKDTDK FKGFCYVEFDEVDSLKEALT YDGALLGDRSLRVDIAEGRK QDKGGFGFRKGGPDDRGFRD DFLGGRGGSRPGDRRTGPPM GSRFRDGPPLRGSNMDFREP TEEERAQRPRLQLKPRTVAT PLNQVANPNSAIFGGARPRE EVVQKEQE
Tissue Specificity The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
Function Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
Protein Name Eukaryotic Translation Initiation Factor 4h
Eif-4h
Williams-Beuren Syndrome Chromosomal Region 1 Protein
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4h antibody
Anti-Eif-4h antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 1 Protein antibody
Anti-EIF4H antibody
Anti-KIAA0038 antibody
Anti-WBSCR1 antibody
Anti-WSCR1 antibody

Information sourced from Uniprot.org

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