| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EGLN2 |
| Gene ID: | 112398 |
| Uniprot ID: | EGLN2_HUMAN |
| Immunogen Region: | 124-223 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 124-223 of human Prolyl hydroxylase PHD1 (EGLN2) (NP_444274.1). |
| Immunogen Sequence: | DGGDAPSPSKRPWARQENQE AEREGGMSCSCSSGSGEASA GLMEEALPSAPERLALDYIV PCMRYYGICVKDSFLGAALG GRVLAEVEALKRGGRLRDGQ |
| Tissue Specificity | Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. |
| Post Translational Modifications | Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation. |
| Function | Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions. Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4. |
| Protein Name | Prolyl Hydroxylase Egln2 Egl Nine Homolog 2 Estrogen-Induced Tag 6 Eit-6 Hph-3 Hypoxia-Inducible Factor Prolyl Hydroxylase 1 Hif-Ph1 Hif-Prolyl Hydroxylase 1 Hph-1 Prolyl Hydroxylase Domain-Containing Protein 1 Phd1 |
| Database Links | Reactome: R-HSA-1234176 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Prolyl Hydroxylase Egln2 antibody Anti-Egl Nine Homolog 2 antibody Anti-Estrogen-Induced Tag 6 antibody Anti-Eit-6 antibody Anti-Hph-3 antibody Anti-Hypoxia-Inducible Factor Prolyl Hydroxylase 1 antibody Anti-Hif-Ph1 antibody Anti-Hif-Prolyl Hydroxylase 1 antibody Anti-Hph-1 antibody Anti-Prolyl Hydroxylase Domain-Containing Protein 1 antibody Anti-Phd1 antibody Anti-EGLN2 antibody Anti-EIT6 antibody |
Information sourced from Uniprot.org