• Western blot analysis of lysates from A-549 cells, using E2F5 Rabbit polyclonal antibody (STJ118482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-E2F5 antibody (197-346) (STJ118482)

SKU:
STJ118482

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E2F5 (197-346) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F5
Gene ID: 1875
Uniprot ID: E2F5_HUMAN
Immunogen Region: 197-346
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 197-346 of human E2F5 (NP_001942.2).
Immunogen Sequence: QLEVPIPEMGQNGQKKYQIN LKSHSGPIHVLLINKESSSS KPVVFPVPPPDDLTQPSSQS LTPVTPQKSSMATQNLPEQH VSERSQALQQTSATDISSAG SISGDIIDELMSSDVFPLLR LSPTPADDYNFNLDDNEGVC DLFDVQILNY
Function Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Protein Name Transcription Factor E2f5
E2f-5
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-1538133
Reactome: R-HSA-2173796
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f5 antibody
Anti-E2f-5 antibody
Anti-E2F5 antibody

Information sourced from Uniprot.org

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