• Western blot analysis of extracts of BT-474 cells, using DUSP10 antibody (STJ111398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of BT-474 cells, using DUSP10 antibody (STJ111398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DUSP10 antibody (1-300) (STJ111398)

SKU:
STJ111398

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP10
Gene ID: 11221
Uniprot ID: DUS10_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DUSP10 (NP_009138.1).
Immunogen Sequence: MPPSPLDDRVVVALSRPVRP QDLNLCLDSSYLGSANPGSN SHPPVIATTVVSLKAANLTY MPSSSGSARSLNCGCSSASC CTVATYDKDNQAQTQAIAAG TTTTAIGTSTTCPANQMVNN NENTGSLSPSSGVGSPVSGT PKQLASIKIIYPNDLAKKMT KCSKSHLPSQGPVIIDCRPF MEYNKSHIQGAVHINCADKI SRRRLQQGKITVLDLISCRE GKDSFKRIFSKEIIVYDEN
Tissue Specificity Expressed in keratinocytes (at protein level). Detected in brain.
Function Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.
Protein Name Dual Specificity Protein Phosphatase 10
Mitogen-Activated Protein Kinase Phosphatase 5
Map Kinase Phosphatase 5
Mkp-5
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-5675221
Reactome: R-HSA-9652817
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 10 antibody
Anti-Mitogen-Activated Protein Kinase Phosphatase 5 antibody
Anti-Map Kinase Phosphatase 5 antibody
Anti-Mkp-5 antibody
Anti-DUSP10 antibody
Anti-MKP5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance