• Western blot analysis of lysates from rat testis, using DNAAF1 Rabbit polyclonal antibody (STJ119020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DNAAF1 antibody (516-725) (STJ119020)

SKU:
STJ119020

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNAAF1 (516-725) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAAF1
Gene ID: 123872
Uniprot ID: DAAF1_HUMAN
Immunogen Region: 516-725
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 516-725 of human DNAAF1 (NP_848547.4).
Immunogen Sequence: VATEGVFVTELDGTRTEDLE TIRLETKETFCIDDLPDLED DDETGKSLEDQNMCFPKIEV ISSLSDDSDPELDYTSLPVL ENLPTDTLSNIFAVSKDTSK AARVPFTDIFKKEAKRDLEI RKQDTKSPRPLIQELSDEDP SGQLLMPPTCQRDAAPLTSS GDRDSDFLAASSPVPTESAA TPPETCVGVAQPSQALPTWD LTAFPAPKAS
Tissue Specificity Mainly expressed in trachea and testis.
Function Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli.
Protein Name Dynein Axonemal Assembly Factor 1
Leucine-Rich Repeat-Containing Protein 50
Cellular Localisation Cell Projection
Cilium
Cytoplasm
Cytoskeleton
Spindle Pole
In Hek293t Cells
It Is Diffusely Cytoplasmic And Concentrates At The Mitotic Spindle Poles
While In Mdck Cells
It Localizes In The Cilium
In Vivo
This Protein Is Probably Restricted To The Cilium
Alternative Antibody Names Anti-Dynein Axonemal Assembly Factor 1 antibody
Anti-Leucine-Rich Repeat-Containing Protein 50 antibody
Anti-DNAAF1 antibody
Anti-LRRC50 antibody

Information sourced from Uniprot.org

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