• Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (STJ23994). Blue: DAPI for nuclear staining.
  • Chromatin Immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (STJ23994) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (STJ23994) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (STJ23994) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (STJ23994) at dilution of 1:200 (40x lens).
  • Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (STJ23994).
  • Western blot analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 antibody (STJ23994) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-DiMethyl-Histone H3-K79 antibody (STJ23994)

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Host: Rabbit
Applications: WB, IHC, IF, IP, ChIP, ChIPseq
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H3.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:50-1:200
IP: 1:50-1:200
ChIP: 1:50-1:200
ChIPseq: 1:50-1:200
Uniprot ID: H31T_HUMAN
Gene ID: 8290
Gene Symbol: H3-4
Immunogen: A synthetic peptide of human DiMethyl-Histone H3-K79
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Tissue Specificity Expressed in testicular cells.
Database Links Reactome: R-HSA-110328
Cellular Localisation Nucleus
Protein Name Histone H3.1t
Histone H3.4
Alternative Names Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody

Information sourced from uniprot.org

6 months standard