• Western blot analysis of extracts of various cell lines, using DEK antibody (STJ111256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DEK antibody (50-160) (STJ111256)

SKU:
STJ111256

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DEK (50-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DEK
Gene ID: 7913
Uniprot ID: DEK_HUMAN
Immunogen Region: 50-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human DEK (NP_003463.1).
Immunogen Sequence: KSLIVEGKREKKKVERLTMQ VSSLQREPFTIAQGKGQKLC EIERIHFFLSKKKTDELRNL HKLLYNRPGTVSSLKKNVGQ FSGFPFEKGSVQYKKKEEML KKFRNAMLKSI
Tissue Specificity Ubiquitous. Expressed at relatively high levels.
Post Translational Modifications Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
Function Involved in chromatin organization.
Protein Name Protein Dek
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-8864260
Reactome: R-HSA-9616222
Cellular Localisation Nucleus
Enriched In Regions Where Chromatin Is Decondensed Or Sparse In The Interphase Nuclei
Alternative Antibody Names Anti-Protein Dek antibody
Anti-DEK antibody

Information sourced from Uniprot.org

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