| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ATP-dependent RNA helicase DDX42 (850-930 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DDX42 |
| Gene ID: | 11325 |
| Uniprot ID: | DDX42_HUMAN |
| Immunogen Region: | 850-930 aa |
| Specificity: | DDX42 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 850-930 |
| Function | ATP-dependent RNA helicase that binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm. |
| Protein Name | Atp-Dependent Rna Helicase Ddx42 Dead Box Protein 42 Rna Helicase-Like Protein Rhelp Rna Helicase-Related Protein Rnahp Sf3b Dead Box Protein Splicing Factor 3b-Associated 125 Kda Protein Sf3b125 |
| Database Links | Reactome: R-HSA-72163 Reactome: R-HSA-72165 |
| Cellular Localisation | Cytoplasm Nucleus Isoform 2: Nucleus Cajal Body Nucleus Speckle |
| Alternative Antibody Names | Anti-Atp-Dependent Rna Helicase Ddx42 antibody Anti-Dead Box Protein 42 antibody Anti-Rna Helicase-Like Protein antibody Anti-Rhelp antibody Anti-Rna Helicase-Related Protein antibody Anti-Rnahp antibody Anti-Sf3b Dead Box Protein antibody Anti-Splicing Factor 3b-Associated 125 Kda Protein antibody Anti-Sf3b125 antibody Anti-DDX42 antibody |
Information sourced from Uniprot.org