• Western blot analysis of extracts of various cell lines, using DAZ1 antibody (STJ27263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DAZ1 antibody (340-530) (STJ27263)

SKU:
STJ27263

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DAZ1 (340-530) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAZ1
Gene ID: 1617
Uniprot ID: DAZ1_HUMAN
Immunogen Region: 340-530
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-530 of human DAZ1 (NP_004072.3).
Immunogen Sequence: NSTISREASTQSSSAAASQG WVLPEGKIVPNTVFVGGIDA RMDETEIGSCFGRYGSVKEV KIITNRTGVSKGYGFVSFVN DVDVQKIVGSQIHFHGKKLK LGPAIRKQKLCARHVQPRPL VVNPPPPPQFQNVWRNPNTE TYLQPQITPNPVTQHVQSAA NPETPNSTISREASTQSSSA AASQGWVLPEG
Tissue Specificity Testis-specific. Expression restricted to premeiotic germ cells, particularly in spermatogonia (at protein level).
Function RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Promotes germ-cell progression to meiosis and formation of haploid germ cells.
Protein Name Deleted In Azoospermia Protein 1
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Nuclear At Some Stages Of Spermatozoide Development
Localizes Both To The Nuclei And Cytoplasm Of Spermatozoide Differentiation
Nuclear In Fetal Gonocytes And In Spermatogonial Nuclei
It Then Relocates To The Cytoplasm During Male Meiosis
Alternative Antibody Names Anti-Deleted In Azoospermia Protein 1 antibody
Anti-DAZ1 antibody
Anti-DAZ antibody
Anti-SPGY antibody

Information sourced from Uniprot.org

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