| Host: | Mouse |
| Applications: | WB/IHC/ICC |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-CRISPR Cas9 (C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 6F7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | 100 ul in PBS + 50% Glycerol and 5 mM Sodium Azide |
| Purification: | This antibody was protein g purified culture from supernatant. |
| Dilution Range: | WB 1:1000IHC 1:1000-1:5000ICC 1:500-1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | C-Term |
| Immunogen: | Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus. |
| Background | CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015). |
Information sourced from Uniprot.org