• Western blot analysis of extracts of various cell lines, using CPNE6 antibody (STJ116597) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CPNE6 antibody (268-557) (STJ116597)

SKU:
STJ116597

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPNE6 (268-557) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPNE6
Gene ID: 9362
Uniprot ID: CPNE6_HUMAN
Immunogen Region: 268-557
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 268-557 of human CPNE6 (NP_006023.1).
Immunogen Sequence: KYRDKKKNYKSSGTVVLAQC TVEKVHTFLDYIMGGCQISF TVAIDFTASNGDPRSSQSLH CLSPRQPNHYLQALRAVGGI CQDYDSDKRFPAFGFGARIP PNFEVSHDFAINFDPENPEC EEISGVIASYRRCLPQIQLY GPTNVAPIINRVAEPAQREQ STGQATKYSVLLVLTDGVVS DMAETRTAIVRASRLPMSII IVGVGNADFSDMRLLDGDDG PLRCPRGVPAARDIVQFVP
Tissue Specificity Widely expressed in the brain. Expressed weakly in the kidney, liver and fetal heart. Expressed in melanocytes.
Function Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Binds phospholipid membranes in a calcium-dependent manner. Plays a role in dendrite formation by melanocytes.
Protein Name Copine-6
Copine Vi
Neuronal-Copine
N-Copine
Database Links Reactome: R-HSA-1483206
Cellular Localisation Cytoplasm
Cell Membrane
Endosome
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Perikaryon
Cell Projection
Dendrite
Mainly Cytoplasmic In Absence Of Calcium
Associated Predominantly With Membranes In Presence Of Calcium
Translocates To The Cell Membrane In A Calcium-Dependent Manner
Colocalized With Transferrin In Intracellular Clathrin-Coated Membrane Vesicles In A Calcium-Dependent Manner
Alternative Antibody Names Anti-Copine-6 antibody
Anti-Copine Vi antibody
Anti-Neuronal-Copine antibody
Anti-N-Copine antibody
Anti-CPNE6 antibody

Information sourced from Uniprot.org

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