STJ72892 (0. 5µg/ml) staining of Mouse Olfactory bulb (lanes1 and 3) and Cerebral cortex (lanes 2 and 4) , comparing wildtype (lanes 3 and 4) with KO mice (lanes 1 and 2). The last lane contains a lysate of HEK293 overexpressing Mouse Cntn4 (lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Gerrald Lodewijk and Peter Burbach, Rudolf Magnus Institute, Utrecht, Netherlands.

Anti-Contactin 4/Big-2-Mouse antibody (160-172) (STJ72892)

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STJ72892-100
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-Contactin 4/Big-2-Mouse (160-172) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 150kDa band observed in Mouse Brain lysates and not in the same lysates from the KO mice (calculated MW of 113kDa according to NP_001103219.1). Recommended concentration: 0.5-1
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CNTN4
Gene ID: 152330
Uniprot ID: CNTN4_HUMAN
Immunogen Region: 160-172
Accession Number: NP_001103219.1; NP_766592.2
Specificity: This antibody is expected to recognize both reported isoforms (NP_001103219.1; NP_766592.2). Reported variants represent identical protein: NP_766592.2, NP_001103221.1
Immunogen Sequence: RRADGKPIARK
Function Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.
Protein Name Contactin-4
Brain-Derived Immunoglobulin Superfamily Protein 2
Big-2
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Alternative Antibody Names Anti-Contactin-4 antibody
Anti-Brain-Derived Immunoglobulin Superfamily Protein 2 antibody
Anti-Big-2 antibody
Anti-CNTN4 antibody

Information sourced from Uniprot.org

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