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Anti-Collagen I antibody [SM0490] (STJA0010490)

Immunohistochemistry analysis of Collagen I. Sample: Rat lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
Immunohistochemistry analysis of Collagen I. Sample: Rat lung, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
Immunohistochemistry analysis of Collagen I. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
Immunohistochemistry analysis of Collagen I. Sample: Human kindey, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
Immunohistochemistry analysis of Collagen I. Sample: Human kindey, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
Immunohistochemistry analysis of Collagen I. Sample: Human liver cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.
Immunohistochemistry analysis of Collagen I. Sample: Rat liver, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.

Anti-Collagen I antibody [SM0490] (STJA0010490)

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STJA0010490

£170.50 - £233.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Collagen I is suitable for use in Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	SM0490
Conjugation:	Unconjugated
Isotype:	lgG2ak
Formulation:	PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol.
Purification:	Affinity purification
Dilution Range:	IHC/IF (H, M, R) 1:500-1:1000
Storage Instruction:	Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.

Gene Symbol:	COL1A1
Gene ID:	1277
Uniprot ID:	CO1A1_HUMAN
Immunogen:	KLH conjugated Synthetic peptide corresponding to Human Collagen I

Post Translational Modifications	Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name	Collagen Alpha-1(I Chain Alpha-1 Type I Collagen
Database Links	Reactome: R-HSA-114604 Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-202733 Reactome: R-HSA-216083 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-3000480 Reactome: R-HSA-430116 Reactome: R-HSA-75892 Reactome: R-HSA-76009 Reactome: R-HSA-8874081 Reactome: R-HSA-8940973 Reactome: R-HSA-8948216 Reactome: R-HSA-9845619 Reactome: R-HSA-9845620 Reactome: R-HSA-9845621 Reactome: R-HSA-9845622 Reactome: R-HSA-9846298
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Collagen Alpha-1(I Chain antibody Anti-Alpha-1 Type I Collagen antibody Anti-COL1A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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